1TF6
CO-CRYSTAL STRUCTURE OF XENOPUS TFIIIA ZINC FINGER DOMAIN BOUND TO THE 5S RIBOSOMAL RNA GENE INTERNAL CONTROL REGION
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12B |
| Synchrotron site | NSLS |
| Beamline | X12B |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-10 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| 格子定数 [Å] | 64.180, 64.712, 78.035 |
| 格子定数 [度] | 90.07, 92.98, 102.95 |
精密化法
| 残基 | 8.000 - 3.100 |
| R因子 | 0.308 |
| Rwork | 0.308 |
| R-free | 0.36300 |
| Structure solution method | MIR |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.005 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.900 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | X-PLOR (3.851) |
Quality characteristics
| Overall | |
| 分解能 [Å] (低) | 25.000 |
| 分解能 [Å] (高) | 3.000 |
| Rmerge_l_obs | 0.128 |
| Total number of observations | 126746 * |
| 独立反射数 | 19034 |
| 完全性 [%] | 77.4 |
| 冗長性 | 6.7 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | COMPLEX WAS CRYSTALLIZED FROM 22.5% PEG 4000, 165 MM NACL, 35 MM SODIUM ACETATE, 3.2 MM DTT, 9.2% (VOL/VOL) GLYCEROL, 1.8 MM NAN3, 1.8 MM CADAVERINE-2HCL, 5.5 MM TRIS-HCL, PH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291.00K |
結晶化試薬
| ID | 結晶ID | 濃度 | 試薬名 | 濃度 (単位) | 詳細 |
| 1 | 1 | 1 | WATER | ||
| 10 | 1 | 2 | WATER | ||
| 11 | 1 | 2 | PEG 4000 | ||
| 2 | 1 | 1 | NACL | ||
| 3 | 1 | 1 | SODIUM ACETATE | ||
| 4 | 1 | 1 | DTT | ||
| 5 | 1 | 1 | GLYCEROL | ||
| 6 | 1 | 1 | NAN3 | ||
| 7 | 1 | 1 | CADAVERINE-2HCL | ||
| 8 | 1 | 1 | TRIS-HCL | ||
| 9 | 1 | 1 | PEG 4000 |
