1TDZ
Crystal Structure Complex Between the Lactococcus Lactis FPG (Mutm) and a FAPY-dG Containing DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9330 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 91.408, 91.408, 141.575 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.18034 |
| Rwork | 0.179 |
| R-free | 0.20697 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nnj |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.709 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.700 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 55781 | |
| <I/σ(I)> | 8.7 | 3.1 |
| Completeness [%] | 99.3 | 100 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | hepes, sodium citrate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | hepes | ||
| 2 | 1 | 1 | sodium citrate |
