1TDZ
Crystal Structure Complex Between the Lactococcus Lactis FPG (Mutm) and a FAPY-dG Containing DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9330 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 91.408, 91.408, 141.575 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.800 |
R-factor | 0.18034 |
Rwork | 0.179 |
R-free | 0.20697 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1nnj |
RMSD bond length | 0.018 |
RMSD bond angle | 1.709 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.19) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 76.700 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 55781 | |
<I/σ(I)> | 8.7 | 3.1 |
Completeness [%] | 99.3 | 100 |
Redundancy | 3.6 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | hepes, sodium citrate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | hepes | ||
2 | 1 | 1 | sodium citrate |