1TDB

STRUCTURES OF THYMIDYLATE SYNTHASE WITH A C-TERMINAL DELETION: ROLE OF THE C-TERMINUS IN ALIGNMENT OF D/UMP AND CH2H4FOLATE

Experimental procedure

Spacegroup name	P 61 2 2
Unit cell lengths	78.800, 78.800, 243.200
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	9999.000 * - 2.670 *
R-factor	0.207 *
Rwork	0.210
RMSD bond length	0.024
RMSD bond angle	4.400
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	2.670 *
Rmerge	0.108 *
Number of reflections	12161 *
Completeness [%]	89.0 *
Redundancy	4.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	phosphate
2	1	drop	C-terminal valine	5 (mg/mL)
3	1	drop	ammonium sulfate	1 (%sat)
4	1	drop	FdUMP	5 (mM)
5	1	reservoir	C-terminal valine	5 (mg/mL)