1TBG
BETA-GAMMA DIMER OF THE HETEROTRIMERIC G-PROTEIN TRANSDUCIN
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-06-07 |
| Detector | FUJI |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 85.100, 94.000, 194.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.100 |
| R-factor | 0.2 |
| Rwork | 0.200 |
| R-free | 0.30600 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.890 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.100 * |
| Rmerge | 0.088 |
| Total number of observations | 258531 * |
| Number of reflections | 88015 |
| Completeness [%] | 96.0 |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 4 * | protein solution is mixed in a 1:1 ratio with well solution * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15-20 (mg/ml) | |
| 10 | 1 | reservoir | glycerol | 5 (%(v/v)) | |
| 11 | 1 | reservoir | PEG4000 | 5 (%(v/v)) | |
| 12 | 1 | reservoir | beta-mercaptoethanol | 15 (mM) | |
| 2 | 1 | drop | Tris-HCl | 1 (mM) | |
| 3 | 1 | drop | 1 (mM) | ||
| 4 | 1 | drop | 5 (mM) | ||
| 5 | 1 | drop | beta-mercaptoethanol | 15 (mM) | |
| 6 | 1 | drop | glycerol | 5-7.5 (%(v/v)) | |
| 7 | 1 | reservoir | fumarate | 10 (mM) | |
| 8 | 1 | reservoir | 10 (mM) | ||
| 9 | 1 | reservoir | 2 (mM) |
