1T9S
Catalytic Domain Of Human Phosphodiesterase 5A in Complex with GMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2003-10-03 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.969, 90.872, 68.991 |
| Unit cell angles | 90.00, 98.07, 90.00 |
Refinement procedure
| Resolution | 69.010 - 2.000 |
| R-factor | 0.17712 |
| Rwork | 0.175 |
| R-free | 0.21235 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1t9r |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.352 |
| Data scaling software | ELVES |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.1.25) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.010 | 2.052 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.124 | 0.558 |
| Number of reflections | 48496 | |
| <I/σ(I)> | 6.4 | 2.8 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | PEG 4000, lithium sulfate, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
