1T8U
Crystal Structure of human 3-O-Sulfotransferase-3 with bound PAP and tetrasaccharide substrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-03-17 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 80.806, 154.525, 91.931 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.950 |
| R-factor | 0.199 |
| Rwork | 0.199 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | h3-OST-3 with bound PAP |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 42327 | |
| <I/σ(I)> | 18.2 | |
| Completeness [%] | 99.9 | 99.4 |
| Redundancy | 3.8 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | micro-seeding | 5.5 | 297 | sodium citrate, ammonium acetate, PEG 4000, PAP, pH 5.5, micro-seeding, temperature 297K |
