1T8P

Crystal structure of Human erythrocyte 2,3-bisphosphoglycerate mutase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BSRF BEAMLINE 3W1A
Synchrotron site	BSRF
Beamline	3W1A
Temperature [K]	100
Detector technology	CCD
Collection date	2003-10-26
Detector	MARRESEARCH
Wavelength(s)	0.9
Spacegroup name	P 1 21 1
Unit cell lengths	38.638, 61.768, 123.333
Unit cell angles	90.00, 94.99, 90.00

Refinement procedure

Resolution	29.950 - 2.500
R-factor	0.2
Rwork	0.200
R-free	0.26600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5pgm
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.560
High resolution limit [Å]	2.500	2.500
Number of reflections	18907
Completeness [%]	96.0	97.2
Redundancy	9.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	20%(w/v) PEG6000, 0.1M Hepes, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K