1T7P
T7 DNA POLYMERASE COMPLEXED TO DNA PRIMER/TEMPLATE,A NUCLEOSIDE TRIPHOSPHATE, AND ITS PROCESSIVITY FACTOR THIOREDOXIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-11 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 106.600, 216.300, 52.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.24 |
| Rwork | 0.240 |
| R-free | 0.27900 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 22.860 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.054 * | 0.151 * |
| Total number of observations | 162012 * | |
| Number of reflections | 60190 * | |
| <I/σ(I)> | 20.8 | 6.5 |
| Completeness [%] | 96.5 | 86.7 |
| Redundancy | 2.7 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.5 * | used to seeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | PEG8000 | 12 (%(w/v)) | |
| 2 | 1 | 1 | ACES | 100 (mM) | |
| 3 | 1 | 1 | ammonium sulfate | 120 (mM) | |
| 4 | 1 | 1 | 30 (mM) | ||
| 5 | 1 | 1 | dithiothreitol | 5 (mM) |
