1T6S
Crystal structure of a conserved hypothetical protein from Chlorobium tepidum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-04-25 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 62 |
Unit cell lengths | 69.420, 69.420, 117.990 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 1.950 |
R-factor | 0.23 |
Rwork | 0.216 |
R-free | 0.25310 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.240 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 500.000 | 1.980 |
High resolution limit [Å] | 1.950 | 1.950 |
Number of reflections | 23532 | |
<I/σ(I)> | 11.6 | |
Completeness [%] | 99.3 | 95.7 |
Redundancy | 5.67 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | ammonium nitrate, sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |