1T6P
Crystal Structure of Phenylalanine Ammonia Lyase from Rhodosporidium toruloides
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 78 |
| Detector technology | CCD |
| Collection date | 2000-03-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.980647 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 105.192, 180.973, 149.215 |
| Unit cell angles | 90.00, 96.95, 90.00 |
Refinement procedure
| Resolution | 25.100 - 2.700 |
| Rwork | 0.194 |
| R-free | 0.25320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1t6j |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.373 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.100 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.076 | 0.310 |
| Number of reflections | 146898 | |
| <I/σ(I)> | 14.14 | 3.87 |
| Completeness [%] | 95.6 | 80.6 |
| Redundancy | 3.17 | 3.17 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | PEG 3000, citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
