1T6M
X-ray Structure of the R70D PI-PLC enzyme: Insight into the role of calcium and surrounding amino acids on active site geometry and catalysis.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Detector technology | CCD |
| Collection date | 2001-12-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.99 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 91.221, 147.725, 96.562 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.300 - 2.107 |
| R-factor | 0.2233 |
| Rwork | 0.223 |
| R-free | 0.23970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.546 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 43.437 |
| High resolution limit [Å] | 2.107 |
| Number of reflections | 37903 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 298 | 30% PEG-3000, 0.2M NH4Cl, 20 mM CHES Buffer, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
