1T6J
Crystal Structure of Phenylalanine Ammonia Lyase from Rhodosporidium toruloides
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2000-08-29 |
Detector | BRANDEIS - B4 |
Wavelength(s) | 0.97866,0.97885,0.96350,0.99473 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 107.860, 107.860, 204.380 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.000 - 2.100 |
R-factor | 0.251 |
Rwork | 0.243 |
R-free | 0.29850 |
Structure solution method | MAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.256 |
Data reduction software | MADNESS |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.089 | 0.360 |
Number of reflections | 75419 | |
<I/σ(I)> | 20.5 | 1.98 |
Completeness [%] | 90.0 | 49.4 |
Redundancy | 6.33 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | PEG 3000, citrate, beta-octylglucoside, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |