1T6B
Crystal structure of B. anthracis Protective Antigen complexed with human Anthrax toxin receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2004-04-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.892 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 88.160, 94.118, 135.597 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.500 |
| R-factor | 0.21009 |
| Rwork | 0.207 |
| R-free | 0.26572 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.649 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 39917 | |
| <I/σ(I)> | 11.5 | 2.4 |
| Completeness [%] | 99.9 | |
| Redundancy | 5.3 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 300 | CHES buffer, PEG400, pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 300K |
