1T64
Crystal Structure of human HDAC8 complexed with Trichostatin A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-02-20 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | P 43 |
Unit cell lengths | 81.014, 81.014, 114.178 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 81.650 - 1.900 |
R-factor | 0.172 |
Rwork | 0.172 |
R-free | 0.22087 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1c3r |
RMSD bond length | 0.010 |
RMSD bond angle | 1.272 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 81.000 | 1.950 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.058 | 0.540 |
Number of reflections | 54547 | |
<I/σ(I)> | 23.8 | 2.5 |
Completeness [%] | 99.3 | 99 |
Redundancy | 4.5 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.7 | 277 | PEG 8000, calcium acetate, imidazole, pH 6.7, VAPOR DIFFUSION, SITTING DROP, temperature 277K |