1T61

crystal structure of collagen IV NC1 domain from placenta basement membrane

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2002-10-27
Detector	MARRESEARCH
Wavelength(s)	0.97471
Spacegroup name	P 21 21 21
Unit cell lengths	73.650, 80.139, 235.420
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.500
R-factor	0.177
Rwork	0.174
R-free	0.20000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.350
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.550
High resolution limit [Å]	1.500	1.500
Number of reflections	215169
<I/σ(I)>	12.4
Completeness [%]	96.0	85

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	298	PEG 20K, dioxane, tris buffer, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K