1T60
Crystal structure of Type IV collagen NC1 domain from bovine lens capsule
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-03-21 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.886 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 127.880, 140.129, 160.692 |
| Unit cell angles | 90.00, 91.26, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.500 |
| R-factor | 0.191 |
| Rwork | 0.189 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m3d |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.380 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 859512 | |
| <I/σ(I)> | 13.9 | 1 |
| Completeness [%] | 96.5 | 97.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 298 | PEG20K, bicine, MPD, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
