1T5O

Crystal structure of the translation initiation factor eIF-2B, subunit delta, from A. fulgidus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-11-24
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	105.575, 110.202, 141.498
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	9.990 - 1.900
R-factor	0.197
Rwork	0.197
R-free	0.24600
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	1.600
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.062	0.536
Number of reflections	121790
<I/σ(I)>	13.8	2.8
Completeness [%]	93.5	91
Redundancy	3.5	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	ammonium dihydrogen phosphate, NaCl, methionine, glycerol, HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	ammonium dihydrogen phosphate, NaCl, methionine, glycerol, HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	ammonium dihydrogen phosphate, NaCl, methionine, glycerol, HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K