1T5E
The structure of MexA
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-03-03 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.979186,0.932209,0.979343 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 130.551, 183.585, 213.314 |
| Unit cell angles | 90.00, 107.38, 90.00 |
Refinement procedure
| Resolution | 95.000 - 3.000 |
| R-factor | 0.2738 |
| Rwork | 0.273 |
| R-free | 0.28460 |
| Structure solution method | MAD |
| RMSD bond length | 1.470 |
| RMSD bond angle | 0.009 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 95.000 | 3.150 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.091 | 0.451 |
| Number of reflections | 189293 | |
| <I/σ(I)> | 9.7 | 2 |
| Completeness [%] | 97.8 | 90.6 |
| Redundancy | 3.8 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 10% PEG 3350, 100mM ammonium sulfate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
