1T4F
Structure of human MDM2 in complex with an optimized p53 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12B |
Synchrotron site | NSLS |
Beamline | X12B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-11-01 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97950 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.030, 43.590, 54.570 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.130 - 1.900 |
Rwork | 0.197 |
R-free | 0.23100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ycr |
RMSD bond length | 0.006 |
RMSD bond angle | 1.100 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNX (2000.1) |
Refinement software | CNX (2000.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.025 | 0.072 |
Number of reflections | 7247 | |
<I/σ(I)> | 36.1 | 9.1 |
Completeness [%] | 88.9 | 56.2 |
Redundancy | 4 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 277 | (NH4)2SO4, Bicine, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |