1T3T
Structure of Formylglycinamide synthetase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F2 |
| Synchrotron site | CHESS |
| Beamline | F2 |
| Temperature [K] | 180 |
| Detector technology | CCD |
| Collection date | 2003-10-23 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 65 |
| Unit cell lengths | 145.806, 145.806, 141.353 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.730 - 1.900 |
| R-factor | 0.188 |
| Rwork | 0.188 |
| R-free | 0.20600 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.200 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.102 | 0.302 |
| Number of reflections | 144593 | |
| <I/σ(I)> | 10 | 4.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 1.7M lithium sulfate, 0.5M ammonium sulfate, 100mM sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
