1T2C
Plasmodium falciparum lactate dehydrogenase complexed with NADH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX14.1 |
Synchrotron site | SRS |
Beamline | PX14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-01-01 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.488 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 81.136, 87.124, 92.212 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.750 - 2.010 |
R-factor | 0.14521 |
Rwork | 0.143 |
R-free | 0.18354 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ldg |
RMSD bond length | 0.024 |
RMSD bond angle | 1.815 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.090 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 21521 | |
<I/σ(I)> | 28.5 | 10.1 |
Completeness [%] | 98.3 | 99.8 |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | MPD, Hepes, Imidazole, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |