1T09
Crystal structure of human cytosolic NADP(+)-dependent isocitrate dehydrogenase in complex NADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F2 |
Synchrotron site | CHESS |
Beamline | F2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-03-25 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9791 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 82.710, 82.710, 308.030 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.940 - 2.700 |
R-factor | 0.21 |
Rwork | 0.210 |
R-free | 0.27000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1lwd |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.750 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.103 | 0.386 |
Number of reflections | 30445 | |
<I/σ(I)> | 10.2 | 6.3 |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 9.1 | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | PEG 20000, MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |