1SZZ
Crystal structure of peptide deformylase from Leptospira Interrogans complexed with inhibitor actinonin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-06-23 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.487, 118.926, 95.761 |
| Unit cell angles | 90.00, 111.53, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.300 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1RN5 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.368 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.510 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Number of reflections | 23337 | |
| Completeness [%] | 85.1 | 35.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 10% PEG1k, PEG8k, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
