1SZS

The structure of gamma-aminobutyrate aminotransferase mutant: I50Q

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	SIEMENS
Temperature [K]	100
Detector technology	AREA DETECTOR
Collection date	2000-10-18
Detector	SIEMENS HI-STAR
Spacegroup name	P 32 2 1
Unit cell lengths	108.100, 108.100, 301.700
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.100
R-factor	0.17
Rwork	0.169
R-free	0.20600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.530
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.100	2.100
Number of reflections	115032
Completeness [%]	95.8	95.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.6	293	HEPES, ammonium sulfate, PLP, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K