1SYQ

Human vinculin head domain VH1, residues 1-258, in complex with human talin's vinculin binding site 1, residues 607-636

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Spacegroup name	H 3 2
Unit cell lengths	141.098, 141.098, 104.920
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.250 - 2.420
R-factor	0.194
Rwork	0.188
R-free	0.23600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.510
High resolution limit [Å]	2.420	2.420
Rmerge	0.179	0.485
Number of reflections	15061
<I/σ(I)>	8.3	3
Completeness [%]	97.6	80.8
Redundancy	18

Crystallization Conditions

crystal ID	method	pH	temperature	details
1