1SYQ
Human vinculin head domain VH1, residues 1-258, in complex with human talin's vinculin binding site 1, residues 607-636
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Spacegroup name | H 3 2 |
Unit cell lengths | 141.098, 141.098, 104.920 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.250 - 2.420 |
R-factor | 0.194 |
Rwork | 0.188 |
R-free | 0.23600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.510 |
High resolution limit [Å] | 2.420 | 2.420 |
Rmerge | 0.179 | 0.485 |
Number of reflections | 15061 | |
<I/σ(I)> | 8.3 | 3 |
Completeness [%] | 97.6 | 80.8 |
Redundancy | 18 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |