1SYK

Crystal structure of E230Q mutant of cAMP-dependent protein kinase reveals unexpected apoenzyme conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	200
Detector technology	IMAGE PLATE
Collection date	2000-03-15
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 42
Unit cell lengths	120.300, 120.300, 58.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.800
Rwork	0.202
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ctp
RMSD bond length	0.008
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.800	2.800
Number of reflections	20296
<I/σ(I)>	10.9	2.6
Completeness [%]	96.8	86.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	MPD, MeOH, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K