1SY3
1.00 A Crystal Structure of D30N Mutant of Nitrophorin 4 from Rhodnius Prolixus Complexed with Nitric Oxide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-12-03 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.900 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 70.431, 42.729, 53.124 |
| Unit cell angles | 90.00, 94.27, 90.00 |
Refinement procedure
| Resolution | 9.680 - 1.000 |
| R-factor | 0.14281 |
| Rwork | 0.142 |
| R-free | 0.15924 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1koi |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.699 |
| Data reduction software | XDISPLAYF |
| Data scaling software | d*TREK |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.600 | 1.020 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.073 | 0.124 |
| Number of reflections | 85604 | |
| <I/σ(I)> | 17.2 | 3.5 |
| Completeness [%] | 95.0 | 99.1 |
| Redundancy | 5.12 | 3.06 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | Ammonium Phosphate 3.2 M, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
