1SVU
Structure of the Q237W mutant of HhaI DNA methyltransferase: an insight into protein-protein interactions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-08-13 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 155.000, 47.000, 122.700 |
| Unit cell angles | 90.00, 121.00, 90.00 |
Refinement procedure
| Resolution | 24.540 - 2.660 |
| R-factor | 0.189 |
| Rwork | 0.189 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1FJX (the protein component) |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.540 | 2.750 |
| High resolution limit [Å] | 2.660 | 2.660 |
| Rmerge | 0.082 | 0.231 |
| <I/σ(I)> | 16.8 | 3 |
| Completeness [%] | 91.6 | 89.1 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 289 | ammonium sulfate, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
