1SVS
Structure of the K180P mutant of Gi alpha subunit bound to GppNHp.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2003-03-15 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.9450 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 79.317, 79.317, 105.141 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.500 |
| Rwork | 0.200 |
| R-free | 0.20890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.284 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.560 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 61531 | |
| Completeness [%] | 94.7 | 94.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293.16 | Ammonium Sulphite, Sodium Acetate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.16K |
