1SUQ
CRYSTAL STRUCTURE OF HIV-1 REVERSE TRANSCRIPTASE (RT) IN COMPLEX WITH JANSSEN-R185545
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-D |
Synchrotron site | APS |
Beamline | 14-BM-D |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 4 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 226.110, 69.470, 104.260 |
Unit cell angles | 90.00, 106.82, 90.00 |
Refinement procedure
Resolution | 19.990 - 3.000 |
R-factor | 0.262 |
Rwork | 0.262 |
R-free | 0.32500 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.800 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS (1.0) |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.050 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.078 | 0.415 |
Number of reflections | 29837 | |
Completeness [%] | 95.0 | 73.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.8 | pH 6.80 |