1SU3

X-ray structure of human proMMP-1: New insights into collagenase action

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Detector technology	CCD
Wavelength(s)	1.28200, 1.28380, 1.0500
Spacegroup name	I 4 2 2
Unit cell lengths	142.746, 142.746, 295.308
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.200
R-factor	0.22422
Rwork	0.223
R-free	0.25233
Structure solution method	MAD
RMSD bond length	0.011
RMSD bond angle	1.269
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.020	2.020
Number of reflections	72595
<I/σ(I)>	27.26	27.26
Redundancy	5.46	5.46

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5		1.5M Li2SO4, 0.1M Hepes, pH 7.5, VAPOR DIFFUSION, SITTING DROP