1STO
CRYSTAL STRUCTURE OF OROTATE PHOSPHORIBOSYLTRANSFERASE
Experimental procedure
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 47.200, 47.200, 216.680 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.000 - 2.600 |
| R-factor | 0.176 |
| Rwork | 0.176 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.500 |
| Phasing software | X-PLOR |
| Refinement software | TNT |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.500 * |
| Rmerge | 0.062 * |
| Number of reflections | 6839 * |
| Completeness [%] | 60.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mM) | |
| 2 | 1 | drop | 65 (%sat) | ||
| 3 | 1 | drop | NaHepes | 70 (mM) | |
| 4 | 1 | drop | 0.05 (mM) | ||
| 5 | 1 | drop | beta-mercapto-ethanol | 10 (mM) | |
| 6 | 1 | reservoir | PEG4000 | 30-40 (%(w/v)) | |
| 7 | 1 | reservoir | NaHepes | 70 (mM) |
