1STC

CAMP-DEPENDENT PROTEIN KINASE, ALPHA-CATALYTIC SUBUNIT IN COMPLEX WITH STAUROSPORINE

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	113
Detector technology	IMAGE PLATE
Collection date	1997-01
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	68.990, 72.820, 76.630
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.300
R-factor	0.208
Rwork	0.208
R-free	0.33300 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1cdk
RMSD bond length	0.012
RMSD bond angle	1.547
Data reduction software	MOSFLM
Data scaling software	Agrovata
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.073 *	0.246 *
Total number of observations	64256 *
Number of reflections	16993
<I/σ(I)>		3
Completeness [%]	92.2	92.3
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.4 *	5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	MES-Bis-Tris	25 (mM)
2	1	drop	octanoyl-N-methylglucamide	1.5 (mM)
3	1	reservoir	methanol	15 (%)