1ST0
Structure of DcpS bound to m7GpppG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-08-17 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9790 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.446, 94.783, 178.328 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.900 |
Rwork | 0.187 |
R-free | 0.23130 |
Structure solution method | MIR |
RMSD bond length | 0.024 |
RMSD bond angle | 2.160 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.065 | 0.393 |
Number of reflections | 79643 | |
<I/σ(I)> | 8.6 | 2.4 |
Completeness [%] | 99.0 | 97.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | 0.1M SODIUM ACETATE, PH5; 10mM YCl3, 10% PEG 8000; 2mM m7GpppG; 10mM MgCl2 , pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |