1SSX
0.83A resolution crystal structure of alpha-lytic protease at pH 8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-01-07 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.785 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 65.910, 65.910, 79.697 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 0.830 |
| R-factor | 0.087 |
| Rwork | 0.086 |
| R-free | 0.09910 |
| Structure solution method | REFINEMENT OF 1TAL.PDB |
| Starting model (for MR) | 1tal |
| RMSD bond length | 0.025 |
| RMSD bond angle | 0.046 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 0.840 |
| High resolution limit [Å] | 0.830 | 0.830 |
| Rmerge | 0.059 | 0.407 |
| Number of reflections | 187431 | |
| <I/σ(I)> | 28.5 | 3 |
| Completeness [%] | 99.6 | 98.8 |
| Redundancy | 4.3 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 1.3M lithium sulfate, 0.02M Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
