1SSG
Understanding protein lids: Structural analysis of active hinge mutants in triosephosphate isomerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-05-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 88.327, 88.327, 163.874 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.960 - 2.900 |
| R-factor | 0.20663 |
| Rwork | 0.205 |
| R-free | 0.24433 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8tim |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.328 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.089 | 0.600 |
| Number of reflections | 16663 | |
| <I/σ(I)> | 25 | 3.4 |
| Completeness [%] | 99.7 | 97.4 |
| Redundancy | 5.11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 294 | Citrate, ammonium sulphate, sodium chloride, 2-phosphoglycolate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
