1SRS
SERUM RESPONSE FACTOR (SRF) CORE COMPLEXED WITH SPECIFIC SRE DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 106.840, 106.840, 76.880 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 3.200 |
| R-factor | 0.228 |
| Rwork | 0.228 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.900 |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.000 |
| High resolution limit [Å] | 3.200 |
| Rmerge | 0.106 |
| Number of reflections | 7629 |
| Completeness [%] | 98.0 |
| Redundancy | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 20 * | pH 6.50, VAPOR DIFFUSION, HANGING DROP, temperature 293.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | PEG 6000 | ||
| 3 | 1 | 1 | NH4 CITRATE | ||
| 4 | 1 | 1 | BIS-TRIS-PROPANE | ||
| 5 | 1 | 1 | MGCL2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein DNA complex | 0.2 (mM) | |
| 2 | 1 | drop | 50-150 (mM) | ||
| 3 | 1 | drop | bis-Tris | 50 (mM) | |
| 4 | 1 | drop | 30 (mM) | ||
| 5 | 1 | drop | PEG6000 | 6 (%) | |
| 6 | 1 | reservoir | 50-150 (mM) | ||
| 7 | 1 | reservoir | bis-Tris | 50 (mM) | |
| 8 | 1 | reservoir | 30 (mM) | ||
| 9 | 1 | reservoir | PEG6000 | 6 (%) |
