1SR4

Crystal Structure of the Haemophilus ducreyi cytolethal distending toxin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9A
Synchrotron site	NSLS
Beamline	X9A
Temperature [K]	113.1
Wavelength(s)	0.92
Spacegroup name	P 21 21 21
Unit cell lengths	71.652, 75.653, 121.637
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	99.000 - 2.000
R-factor	0.182
Rwork	0.182
R-free	0.21400
Structure solution method	SAD
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000	2.070
High resolution limit [Å]	2.000	2.000
Number of reflections	45190
Completeness [%]	99.6	98.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	296.15	15-25% PEG MME 5000, 25-30% glycerol, 0.1M imidazole, 2mM DTT, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 296.15K