1SQL
Crystal structure of 7,8-dihydroneopterin aldolase in complex with guanine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-06-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.542 |
| Spacegroup name | P 1 |
| Unit cell lengths | 63.550, 84.220, 89.050 |
| Unit cell angles | 90.14, 89.99, 76.17 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| Rwork | 0.204 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dhn |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.090 | 0.030 |
| Number of reflections | 86882 | |
| Completeness [%] | 95.5 | |
| Redundancy | 2.7 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 293 | Tris, PEG 2000 MME, C-HEGA-11, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
