1SQD
Structural basis for inhibitor selectivity revealed by crystal structures of plant and mammalian 4-hydroxyphenylpyruvate dioxygenases
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-11-20 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 77.400, 83.600, 63.500 |
| Unit cell angles | 90.00, 102.50, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.800 |
| R-factor | 0.226 |
| Rwork | 0.216 |
| R-free | 0.26600 |
| Structure solution method | MIR |
| RMSD bond length | 0.025 |
| RMSD bond angle | 0.041 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | d*TREK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.041 | 0.269 |
| Number of reflections | 187153 | |
| Completeness [%] | 98.9 | 89.2 |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 298 | PEG 4000, isopropanol, Bis-tris, potassium chloride, cobalt chloride, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
