1SPS

BINDING OF A HIGH AFFINITY PHOSPHOTYROSYL PEPTIDE TO THE SRC SH2 DOMAIN: CRYSTAL STRUCTURES OF THE COMPLEXED AND PEPTIDE-FREE FORMS

Experimental procedure

Spacegroup name	P 41
Unit cell lengths	93.300, 93.300, 55.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 2.700
R-factor	0.18
Rwork	0.180
RMSD bond length	0.014
RMSD bond angle	3.200
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000 *
High resolution limit [Å]	2.700 *
Rmerge	0.067 *
Total number of observations	19885 *
Number of reflections	11714 *
Completeness [%]	89.1 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	HEPES	10 (mM)
2	1	drop	EDTA	5 (mM)
3	1	drop	dithiothreitol	5 (mM)
4	1	drop	PEG4000	10 (%)
5	1	reservoir	MPD	30 (%)