1SOZ
Crystal Structure of DegS protease in complex with an activating peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9393 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 205.984, 142.710, 41.167 |
| Unit cell angles | 90.00, 89.24, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.400 |
| R-factor | 0.214 |
| Rwork | 0.213 |
| R-free | 0.27200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | DegS uncomplexed |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.660 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 42042 | |
| Completeness [%] | 91.8 | 94.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 292 | PEG 6000, MPD, HEPES, magnesium chloride, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
