1SOR
Aquaporin-0 membrane junctions reveal the structure of a closed water pore
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ELECTRON MICROSCOPE |
| Source details | Tecnai T20 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-01-28 |
| Detector | Gatan 2K x 2K |
| Spacegroup name | P 4 2 2 |
| Unit cell lengths | 65.500, 65.500, 160.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.250 - 3.000 |
| R-factor | 0.299 |
| Rwork | 0.299 |
| R-free | 0.33800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1j4n |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.000 |
| Phasing software | MOLREP (V. 7.4.03) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.500 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.160 | 0.540 |
| Number of reflections | 6635 | |
| Completeness [%] | 88.0 | 82 |
| Redundancy | 6.7 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 6 | 298 | magnesium chloride, sodium chloride, MES, sodium azide, DTT, pH 6, MICRODIALYSIS, temperature 25K |
