1SON
ADENYLOSUCCINATE SYNTHETASE IN COMPLEX WITH THE NATURAL FEEDBACK INHIBITOR AMP
Experimental procedure
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-10-13 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 70.500, 70.500, 201.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.550 |
| R-factor | 0.187 |
| Rwork | 0.187 |
| Structure solution method | RIGID BODY REFINEMENT |
| Starting model (for MR) | 1soo |
| RMSD bond length | 0.011 |
| RMSD bond angle | 24.350 * |
| Data reduction software | XDS |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 | 2.620 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.086 | 0.400 |
| Number of reflections | 17293 | |
| Completeness [%] | 99.7 | 99.2 |
| Redundancy | 6.7 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.7 * | 20-25 * | pH 8.6 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 19 (mg/ml) | |
| 2 | 1 | drop | imidazole | 20 (mM) | |
| 3 | 1 | drop | succinate | 75 (mM) | |
| 4 | 1 | drop | 2-mercaptoethanol | ||
| 5 | 1 | reservoir | PEG3350 | 23-25 (%(w/v)) |
