1SO0
Crystal structure of human galactose mutarotase complexed with galactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 275 |
| Detector technology | AREA DETECTOR |
| Collection date | 2004-02-10 |
| Detector | SIEMENS HI-STAR |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 61.000, 68.700, 98.900 |
| Unit cell angles | 107.70, 98.40, 102.70 |
Refinement procedure
| Resolution | 30.000 - 2.300 |
| R-factor | 0.169 |
| Rwork | 0.167 |
| R-free | 0.19800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1snz |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.400 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | AMoRE |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 61156 | |
| <I/σ(I)> | 10.8 | 2.8 |
| Completeness [%] | 94.7 | 77.5 |
| Redundancy | 2.1 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | batch | 6 | 275 | PEG 8000, sodium chloride, lithium chloride, galactose, MES, pH 6.0, batch, temperature 275K |
