1SM2
Crystal structure of the phosphorylated Interleukin-2 tyrosine kinase catalytic domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-28 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.488 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 125.100, 74.400, 78.900 |
| Unit cell angles | 90.00, 93.90, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.223 |
| Rwork | 0.214 |
| R-free | 0.29200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.100 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.072 | 0.527 |
| Number of reflections | 29903 | |
| <I/σ(I)> | 9.6 | 1.5 |
| Completeness [%] | 93.6 | 95.1 |
| Redundancy | 1.8 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 293 | Ammonium Sulfate, Sodium citrate, Magnesium acetate, DTT, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
