1SL2

Ternary 5' complex of T7 DNA polymerase with a DNA primer/template containing a cis-syn thymine dimer on the template and an incoming nucleotide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2003-07-10
Spacegroup name	P 21 21 2
Unit cell lengths	104.983, 212.599, 51.945
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.300
R-factor	0.246
Rwork	0.246
R-free	0.27900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	52685
<I/σ(I)>	30.4	5.88
Completeness [%]	93.3	88.6
Redundancy	27.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5		VAPOR DIFFUSION, HANGING DROP, pH 7.5