1SKW

Binary 3' complex of T7 DNA polymerase with a DNA primer/template containing a disordered cis-syn thymine dimer on the template

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-07-20
Spacegroup name	P 21 21 2
Unit cell lengths	104.718, 211.493, 52.187
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.300
R-factor	0.239
Rwork	0.239
R-free	0.27800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	51467
<I/σ(I)>	21.2	2.6
Completeness [%]	99.3	97.9
Redundancy	24.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5		VAPOR DIFFUSION, HANGING DROP, pH 7.5