1SKM
HhaI methyltransferase in complex with DNA containing an abasic south carbocyclic sugar at its target site
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-10-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.1 |
| Spacegroup name | H 3 2 |
| 格子定数 [Å] | 95.681, 95.681, 315.683 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 24.530 - 2.200 |
| R因子 | 0.183 |
| Rwork | 0.183 |
| R-free | 0.22100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | CAD4 |
| Data scaling software | SCALEPACK |
| Phasing software | GLRF |
| Refinement software | CNS |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 24.530 | 2.250 |
| 分解能 [Å] (高) | 2.200 | 2.200 |
| Rmerge_l_obs | 0.179 | |
| 独立反射数 | 23477 | |
| <I/σ(I)> | 18.4 | |
| 完全性 [%] | 81.9 | 36.9 |
| 冗長性 | 2.9 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | PEG 6000, sodium cacodylate, magnesium acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
結晶化試薬
| ID | 結晶ID | 濃度 | 試薬名 | 濃度 (単位) | 詳細 |
| 1 | 1 | 1 | PEG 6000 | ||
| 2 | 1 | 1 | sodium cacodylate | ||
| 3 | 1 | 1 | magnesium acetate |
