1SJD
x-ray structure of o-succinylbenzoate synthase complexed with n-succinyl phenylglycine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-04-07 |
| Wavelength(s) | 0.6525 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 215.200, 215.200, 257.600 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.870 |
| R-factor | 0.202 |
| Rwork | 0.196 |
| R-free | 0.24100 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1sjb |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | d*TREK |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.940 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Number of reflections | 186822 | |
| <I/σ(I)> | 40.1 | 3.3 |
| Completeness [%] | 99.7 | 99.2 |
| Redundancy | 10.1 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | batch | 8 | 298 | PEG 8000, HEPES, MgCl2, n-succinylphenylglycine, pH 8.0, batch, temperature 298K |
